Development and evaluation of an enzyme-linked immunosorbent assay based on recombinant TgSRS2 for serodiagnosis of Toxoplasma gondii infection in cats

Yang GAO; Huanping GUO; Paul Franck ADJOU MOUMOUNI; Mingming LIU; Jixu LI; Artemis EFSTRATIOU; Eloiza May GALON; Rochelle Haidee YBANEZ; Tatsunori MASATANI; Takehisa SOMA; Xuenan XUAN

doi:10.1292/jvms.20-0231

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Development and evaluation of an enzyme-linked immunosorbent assay based on recombinant TgSRS2 for serodiagnosis of Toxoplasma gondii infection in cats

Yang GAO, Huanping GUO, Paul Franck ADJOU MOUMOUNI, Mingming LIU, Jixu LI, Artemis EFSTRATIOU, Eloiza May GALON, Rochelle Haidee YBANEZ, Tatsunori MASATANI, Takehisa SOMA, Xuenan XUAN

著者情報

Yang GAO
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
Huanping GUO
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
Paul Franck ADJOU MOUMOUNI
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
Mingming LIU
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
Jixu LI
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
Artemis EFSTRATIOU
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
Eloiza May GALON
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
Rochelle Haidee YBANEZ
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine
Tatsunori MASATANI
Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima University
Takehisa SOMA
Veterinary Diagnostic Laboratory, Marupi Lifetech Co., Ltd.
Xuenan XUAN
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine

キーワード: cat, enzyme-linked immunosorbent assay, SAG1-related sequence 2 of Toxoplasma gondii, serodiagnosis, toxoplasmosis

ジャーナルオープンアクセス早期公開

論文ID: 20-0231

DOI https://doi.org/10.1292/jvms.20-0231

この記事には本公開記事があります。

The final version of this article is now available: Vol. 82 (2020), No. 11 pp. 1662-1665

詳細

抄録

An enzyme-linked immunosorbent assay (ELISA) based on recombinant SAG1-related sequence 2 of Toxoplasma gondii (rTgSRS2) was developed to detect toxoplasmosis in cats. The specificity and sensitivity of rTgSRS2 ELISA were confirmed using a series of serum samples from T. gondii-experimentally infected mice. A total of 76 field samples from cats were examined by the developed ELISA. The rTgSRS2 ELISA showed a good diagnostic performance characterized by high concordance (88.16) and kappa value (0.76) with latex agglutination test (LAT). The sensitivity and specificity of the test were 92.68% and 82.86%, respectively. These results suggest that the ELISA based on rTgSRS2 could be a useful tool for serodiagnosis of T. gondii infection in cats.

責任著者(Corresponding author)