Potential shortening of testing time by combination of loop-mediated isothermal amplification and enrichment culture for detection of Salmonella spp. in cattle farms

Abstract

To prevent the spread of bovine salmonellosis on farms, minimizing the detection time is an effective approach. At present, Salmonella spp. is typically detected via conventional culture methods involving an enrichment culture process taking several days. We attempted rapid detection of individual feces and environmental samples spiked with Salmonella Typhimurium or S. Dublin employing a loop-mediated isothermal amplification kit for detecting Salmonella spp. Notably, in comparison with conventional culture methods, the use of 18-hr enrichment cultures as samples can detect Salmonella spp. in feces and environment at least 24 and 48 hr earlier. Additional validation using clinical samples is necessary.