Abstract
1. As the medium which I adopted in this experiment contains no beef protein, partial reaction due to meat does not occur.
2. Rabbits which received successive injections of peptone do not show any development of specific precipitins in their serum.
3. It is possible to obtain precipitins which react only with the protein of B. chauvoei, by treating rabbits with liver-peptone culture of this organism.
4. The negative results in my experiments probably depend upon the other disease which is apparently analogous with the black-leg from biological points of view.
5. The precipitin reaction is serviceable for differentiation of B. chauvoei from B. oedematis maligni, B. tetani, B. welchii and the bacillus of chicken diarrhoea (Nakamura).
