The Japanese Journal of Veterinary Science
Online ISSN : 1881-1442
Print ISSN : 0021-5295
Plaque Assay of Bovine Coronavirus in BEK-1 Cells
Norio HIRANOYasufumi SADAKotaro TUCHIYAKatsuhiko ONOToshiaki MURAKAMI
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Volume 47 (1985) Issue 4 Pages 679-681

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Abstract

The plaque formation of Japanese isolate of bovine coronavirus was studied in BEK-1 cell monolayers. The isolate readily produced clear plaques in 3 days. The plaque numbers obtained were directly proportional to the virus concentration. The procedure of plaque assay was simple, sensitive and reproducible.

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