Plaque Assay of Bovine Coronavirus in BEK-1 Cells
1985 Volume 47 Issue 4 Pages 679-681
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1985 Volume 47 Issue 4 Pages 679-681
The plaque formation of Japanese isolate of bovine coronavirus was studied in BEK-1 cell monolayers. The isolate readily produced clear plaques in 3 days. The plaque numbers obtained were directly proportional to the virus concentration. The procedure of plaque assay was simple, sensitive and reproducible.
