Serum C-Reactive Protein (CRP) in Horses: The Effect of Aging, Sex, Delivery and Inflammations on Its Concentration

Abstract

The serum concentration of C-reactive protein (CRP) in horses was measured by single radial immunodiffusion (SRID) using rabbit anti-equine CRP serum to evaluate the significance of CRP as an acute-phase reactive protein. In serum samples of clinically normal newborn thoroughbred foals before being given colostrum, serum CRP was not detectable (≤1 μg/ml). The serum CRP concentration was found to increase quickly and reach a peak level of approximately 14.1 μg/ml in 12-month-old horses. Then, it decreased gradually to reach a low value of 5.4 μg/ml in the 4-year-old. In horses over 5-year-old, serum CRP concentration was stable at values of 7 to 8 μg/ml. In mares during the peri-natal period, serum CRP concentration decreased at 2 months before delivery, showed moderate changes just before and after delivery, and then increased to relatively high level by 2 months after delivery. The concentration of serum CRP was found to be increased at 24 hours after experimentally inflammatory stimuli in adult horses, and reached peak values of 3 to 6 times as high as the base line values at 3 to 5 days. Serum CRP concentration returned to base line values by 2 to 3 weeks after treatments. It is clear from these data that CRP is one of the acute-phase reactive proteins in horses.