Increased Nuclear Type II Estradiol Receptor Concentrations in Rat Spleen during the Course of Pregnancy

Abstract

Estradiol receptors are classified into type I and type II receptors by their affinity and capacity for estradiol binding. The type II receptors are thought to be significant in the suppression of immune response, such as in the pregnancy-associated immunosuppression. The present study was undertaken to show the presence of type II receptors in rat spleen and to examine the change of receptor distribution after estradiol administration and during pregnancy. Scatchard analysis revealed that the type II receptors were present in rat spleen, and dissociation constants were estimated to be 3.23×10^-9 M for cytosol and 4.29×10^-9 M for nuclei. The receptors possessed specificity for estradiol and diethylstilbestrol, but not for promegestone, methyltrienolone and dexamethasone. Administration of estradiol to rats resulted in the increase of nuclear receptor concentrations with concomitant decrease of cytosolic concentrations. During pregnancy, the receptor concentrations were increased in the nuclear fraction, but were not significantly changed in the cytosolic fraction. The dissociation constants of the receptors in pregnant rdt spleen (4.77×10^-9 M for cytosol and 7.20×10^-9 M for nuclei) were similar to those in the non-pregnant control, suggesting the quantitative change of the receptors during pregnancy.