Abstract
An analytical method for measurement of tetrahydrofolate (THF) and N5-methyltetrahydrofolate (5MF) in pig plasma by the high performance liquid chromatography and electrochemical detection is described. The plasma sample was deproteinized by perchloric acid and the supernatant was analyzed using the following conditions; (a)phenyl bonded phase column as an analytical column; (b)mobile phase consisting of 20mM acetate buffer (pH 3.6) containing 0.1mM EDTA and acetonitrile (96.5:3.5, v/v); (c)an applied potentialof +300mV. Under the above condition, both peaks of THF and 5MF in the plasma were well separated. The detection limits of THF and jMF were 0.15 and 0.13ng/ml, respectively, at S/N=3. The recoveries of THF and 5MF from the plasma spiked with standard THF and 5MF were 77.6±2.1% and 83.0±1.7% (mean±S.D.), respectively.
