Construction of Recombinant Infectious Laryngotracheitis Virus Expressing the LacZ Gene of E. coli with Thymidine Kinase Gene

Hiroshi OKAMURA; Masashi SAKAGUCHI; Takashi HONDA; Akira TANENO; Kazuo MATSUO; Shinji YAMADA

doi:10.1292/jvms.56.799

Journal of Veterinary Medical Science

Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250

Construction of Recombinant Infectious Laryngotracheitis Virus Expressing the LacZ Gene of E. coli with Thymidine Kinase Gene

Hiroshi OKAMURA, Masashi SAKAGUCHI, Takashi HONDA, Akira TANENO, Kazuo MATSUO, Shinji YAMADA

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Keywords: ILTV-CE strain, recombinant ILTV, TK gene

JOURNAL FREE ACCESS

1994 Volume 56 Issue 4 Pages 799-801

DOI https://doi.org/10.1292/jvms.56.799

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Abstract

We constructed the recombinant infectious laryngotracheitis virus (ILTV), CE strain, containing the LacZ gene of E. coli in the thymidine kinase gene. The growth property of the recombinant virus was almost the same as parental CE strain in chicken embryo fibroblasts. The recombinant CE strain of ILTV could be used as a live vaccine vector.

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