Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250
Detection of Serum Antibody against Bovine Coccidiosis by Using Eimeria tenella Antigen
Tatsuya UCHIDAHASBULLAHYutaka NAKAIKeiji OGIMOTO
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1995 Volume 57 Issue 1 Pages 169-171

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Abstract
Antigens of Eimeria tenella sporulated oocysts were applied to detect serum antibody of calf infected with Eimeria. Polypeptides of E. tenella oocyst were strongly proved with the infected bovine serum in immunoblotting. The infected serum also showed higher absorbances than that of normal serum in enzyme linked immunosorbent assay (ELISA) using E. tenella oocyst antigen. Suitable conditions for the ELISA were obtained by using soluble antigen of E. tenella oocysts at 2.5μg/ml and bovine serum at 200 fold dilution.
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© The Japanese Society of Veterinary Science
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