Serum Vitamin A and Vitamin E in Japanese Black Fattening Cattle in Miyazaki Prefecture as Determined by Automatic Column-Switching High Performance Liquid Chromatography

Abstract

Japanese Black fattening cattle in Miyazaki prefecture, Japan were examined for serum vitamin A (V.A) and vitamin E (V.E) by automatic column-switching high performance liquid chromatography with automated deproteinization. Results indicated that most Japanese Black fattening cattle in Miyazaki prefecture may be provided with V.A supplement and diets including little V.E, moderate β-carotene and V.A during the early fattening stage, and diets including little β-carotene, V.A and V.E during the middle and later fattening stages. Therefore, monitoring serum V.A and V.E in Japanese Black fattening cattle throughout the fattening period seems necessary for farmers in Miyazaki prefecture to avoid economic loss attributable to these deficiencies.