Hydrophytes may play an important role in sewage disinfection in constructed wetlands

抄録

Double agar overlay (DAL) method was used to evaluate the inhibition of hydrophytes extracts on coliphages growth by the plaque forming unit (PFU) assay. The present research investigated allelopathic effects of extracts from five hydrophytes leaf, Polygonum hydropiper, Polygonum orientale, Phragmites communis, Arundo donax and Typha latifolia on two coliphages, T4 and f2. The results showed that different hydrophytes inhibit coliphage (T4 and f2) to various extents, and the inhibition of extracts from P. hydropiper was more effective on coliphage T4 and f2 than other hydrophytes. When the concentration of extracts was 0.5g·L^-1, the inhibition rate of P. hydropiper leaf on coliphage T4, f2 was 91%, 93%, respectively. The EC₅₀ of P. hydropiper leaf on coliphage T4 was 10mgmiddot;L^-1, and the EC₅₀ on coliphage f2 was 12.6mgmiddot;L^-1. The EC₅₀ on coliphage T4, f2 of T. latifolia leaf was 16 mgmiddot;L^-1, 20 mgmiddot;L^-1, respectively. The P. hydropiper showed the greatest inhibition on coliphage. The allelochemicals produced by P. hydropiper should be isolated and evaluated in the following research. More research should be done to evaluate potential use of P. hydropiper in wastewater virus controlling.