During the long term cultivation, it is known that linear algae grow in or aritificial Spirulina culture. Numerous experiments were carried out to investigate a method of removing linear algae from the Spirulina culture. It was found that linear algae in the Spirulina culture could be removed when inoculated Spirulina were inthe stationary phase or in the later period of linear growth phase if CaCl2 concentration in themedium was reduced by one third from that of a normal medium.