実験的家兎肺膿瘍にかんする基礎的研究

抄録

Solitary lung abscess was produced by inoculating intrabronchially Staphylococcus aureus in Freund's incomplete adjuvant through a tube inserted perorally to one lung of a rabbit which had been sensitized with killed Staphylococcus aureus in Freund's incomplete adjuvant. After the inoculation the followings were investigated at several times; macroscopic and histological findings of lung abscess, viable cell counts in the various tissues of the lung, and body temperature and peripheral leukocytes counts of the rabbits with lung abscess.
The results obtained were as follows.
1) The lung abscess was produced in 48.3% of rabbits inoculated 5 mg of Staphylococcus aureus and in 37.9% of those inoculated 10 mg.
2) The lung abscess produced by above-mentioned method was pathologically similar to that in man. The inflammatory changes were most remarkable two weeks after the inoculation.
3) Viable cell counts one week after the inoculation in the lesions of the rabbits inoculated 5 mg of Staphylococcus aureus were as follows: approx. 10⁷CFU/g in the pus, 10⁵CFU/g in the wall and 10⁴CFU/g in the surrounding tissue of the abscess. Viable cell counts two weeks after the inoculation were almost same as those one week after. Then, the viable cells decreased gradually to almost nil 8 weeks after the inoculation.
4) The big differences were observed among the rabbits regarding body temperature, leukocytes counts and pseudoeosinophil counts. Accordingly, it seemed difficult to judge the couse of disease by observing these changes.
5) Infiltration of pseudoeosinophils diminished and viable cell counts decreased in the surrounding tissue of the abscess by administering antibiotics for six days from the 8th day after the inoculation of Staphylococcus aureus. As a result fundamental studies on the chemotherapy of pulmonary infection are possible by using this animal model of lung abscess, are surggested.