トルコギキョウに発生したトマト黄化葉巻ウイルス・マイルド系統の配列解析

抄録

In March 2011, Eustoma (Eustoma grandiflorum) plants showing symptoms of a virus-like disease, such as leaf curling, yellowing, and abscised flowers, were found in Ise City, Mie Prefecture. Total DNA extracted from several leaf samples of symptomatic plants was subjected to diagnostic PCR analysis using primers specific to Tomato yellow leaf curl virus. All tested samples were positive for the Mld strain of TYLCV. We amplified the full-length viral genome DNA by rolling circle amplification followed by PCR, and the amplicons were cloned and sequenced. The complete genome sequence of all clones was 2,768 nucleotides in length and had 95% sequence identity with representative TYLCV isolates belonging to the Mld strain originating from tomato plants in Japan. By following the species demarcation criteria of ICTV, the isolate was defined as and grouped in the Mld strain. This is the first report of the complete nucleotide sequence of the Mld strain of TYLCV isolated from the Eustoma plant.