電場刺激法による歯周組織再生に関する実験的研究 : 吸収性膜使用による GTR 法との併用

抄録

Use of electrical stimulation to promote the regeneration of periodontal tissue is effective in periodontal studies, but the volume of periodontal tissue regeneration by electrical stimulation was insufficient. Therefore, It was decided to use the technique of guided tissue regeneration (GTR) method for keeping the space of periodontal regeneration by use of poly (D, L-lactic acid-co-glycolic acid) ; (D, L-LA content : 76 mole%) with electrical stimulation for periodontal tissue regeneration. For the electrical stimulation method, the capacitively coupled electrical field (CCEF) method, which can be applied non-invasively was selected. The purpose of this study was to compare to the effectiveness of the GTR method with electrical stimulation to the GTR method alone, in periodontal regeneration to classIII furcation involvement. As experimental periodontitis was induced bilaterally in mandibular third premolars in dogs, flap surgery was performed and all defects were covered with poly (D, L-lactic acid-co-glycolic acid) ; (D, L-LA content : 76 mole%), so as to prevent soft tissue from proliferating into the defects. After 8 weeks, CCEF signal (sine wave, 60 kHz, 5 V peak to peak) was applied laterally for 4 weeks. Calcein bone labelling compounds were administered to mark new bone and cementum formation. After the application of CCEF signal, the dogs were sacrificed. Decalcified and non-decalcified sections were prepared for histological observation, and the following parameters were measured : the percentage of the length of new bone crest height to defect height (BC) ; the percentage of the distance of new cementum to the distance around the notch to notch (cC) ; the percentage of the area of new bone to that of the defect (BF) ; the length from the top of the soft tissue to the top of the furcation fornix (GM). The results were as follows : 1) BC was higher in value in the stimulated site than in the control site (stimulated site : 65.68±17.84% ; control site : 50.39±14.24%). 2) BF was markedly higher in value in the stimulated site than in the control site (stimulated site : 46.53±20.21% ; control site : 26.75±10.61%). 3) cC and GM did not show identical tendencies. 4) If the epithelium invaded into the defect at the stimulated site, new bone were formed actively. 5) According to the these results, it was suggested that the treatment method of combining the CCEF method with GTR method is useful as a reconstructive periodontal therapy in class III furcation involvement.