Experimental Investigation of Chromatic Aberration in Electron Microscope and Observation of Thick Specimen

Abstract

A experimental investigation of Chromatic aberration in the magnetic electron microscope has been carried out. And by combining the properly selected magnetic lenses an electron microscope with small Chromatic aberration has been developed. This success has led to the attainment of the high resolution on the condition that percentage fluctuation of accelerating voltage is about 2×10^-3. It has enablled also to observe the internal stuctures of thick specimens. using 50kv voltage supply. Even for specimens of 0.3μ0.5μ thick tissues, for instance electron micrographs of high quality can be obtained by means of this instrument.