In the present paper the mass transfer of a substance such as oxygen in a pulsating flow through a two dimensional stenosed channel has been studied. In the experiment both local and mean mass transfer at the wall behind the stenosis are measured by an electrochemical method. Measurements are conduced in a Reynolds number 150. Schmidt numbers 1100 and 1600 and a non-dimensional pulsating frequency range 1.0-7.0. The mass transfer is numerically estimated by the continuity equation, the vorticity transferred equation and the diffusion equation. The local mass transfer at the region just behind the stenosis is lower than that away from the stenosis, and its pulsating amplitude at the former region is smaller than that at the latter region. From the frequency characteristics of the relative mass transfer, the time when the mass transfer at the region just behind the stenosis decreases is remarkably affected by the pulsating frequency.