モノクローナル抗体を利用したTAS-ELISAによるダイズわい化ウイルス (SbDV) 保毒アブラムシの検定

抄録

Enzyme-linked immunosorbent assay (TAS-ELISA) using monoclonal antibodies was adapted to efficiently detect Soybean dwarfvirus (SbDV) in aphids. We used monoclonal antibodies produced using the SbDV-YS strain transmitted specifically by the aphid vector Aulacorthum solani and using the SbDV-YP strain transmitted by Acyrthosiphon pisum. Clarification of aphid extracts with a mixture of chloroform and n-butanol raised absorbance of positive reactions, and SbDV-YS and YP were detected in a single nymph allowed a 1-day acquisition access to infected soybean or white clover leaves. ELISA using these monoclonal antibodies is useful for surveying viruliferous aphids around soybean fields.