We investigated the susceptibility of four populations of soybean pod borer, Leguminivora glycinivorella, in the Tohoku region to the following six insecticides: diamides (chlorantraniliprole, flubendiamide, and tetraniliprole), organic phosphorus (methylerythritol phosphate), synthetic pyrethroid (etofenprox), and neonicotinoid (acetamiprid). The susceptibility to diamides was investigated using insect-dip and pod-dip methods, and the susceptibility to other insecticides was examined using only the insect-dip method. Corrected mortality using Abbott’s formula for the three diamide insecticides was higher in the pod-dip method than in the insect-dip method, excluding the chlorantraniliprole treatment of the Iwate population (insect-dip method, 76%; pod-dip method, 64%). These results could be attributed to the mode of mechanism of diamides, i.e., oral toxicity. Similarly, organic phosphorus, synthetic pyrethroid, and neonicotinoid were highly toxic. Conversely, the control (water-treated) also demonstrated high mortality, irrespective of the insect-dip or pod-dip method, reflecting a higher mortality of first-instar larvae than the treatment. Therefore, in the insecticide susceptibility test using first-instar larvae of soybean pod borer, the results should be evaluated by considering the high mortality rate in the control. In conclusion, susceptibility to insecticides in the four Tohoku regions has not decreased.
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