抄録
An antitumor agent against Yoshida Sarcoma was produced in rat bone marrow cells which were sensitized in vitro with the medium containing the supernatant fluid of medium of X-irradiated Yoshida sarcoma cells.
From 2, 000R to 4, 000R were optimal doses to release an antigenic cellular agent from irradiated Yoshida Sarcoma cells. And a small amount of serum component was necsesary in culture medium of the tumor cells to obtain the antigenic agent.
Specific activity of antigenic activity was determined between tumor cells and normal tissue cells using C3H/He mouse mammary carcinoma and C3H/He mouse kidney cells.
Lymph node cells sensitized with the tumor cell-antigenic-agent did not inhibit the cell growth of normal kidney cells while inhibit the cell growth of target mouse mammary carcinoma cells.
Effect of sensitized lymph node cells and X-Ray irradiation against tumor growth of C3H/He mouse mammary carcinoma which were inoculated into thigh muscle determined.
The tumors were reduced remarkably when they were treated with sensitized lymph node cells and X-Ray irradiation.
