Standard procedure for determination of A subgroups was established by agglutinability of red cells using Falcata japonica lectin besides the reaction with human anti-A, eel anti-H sera and Dolichos biflorus lectin. Family example of Aint which members demonstrate difference in A antigen strength of erythrocytes and in activity of serum A-enzyme was found by the standarized procedure.A2> and A3 red cells did not react with Dolichos biflorus while Falcata japonica agglutinated A2 red cells but not A3 red cells at a titier of 1 : 64. There are dispersions in agglutinability of A2 red cells with Falcata japonica. Weaker α-N-acetylgalactosaminyltransferase activity than that of A1 was demonstrated in Aint and A2 sera by the conversion of group O red cells into A active cells using papain treated cells as acceptor and anti-A immune serum for agglutinability test. A-transferase activity which converts O red cells into A active cells at the presence of UDP-N-acetylgalactosamine was found in group B sera.