抄録
Three continuous cell lines (GMC-N, GMC-U GMC-Y) have been established from different primary breast cancers. To remove contaminated fibroblasts from epithelial cells, floating cells in the primary culture were serially transferred to a new dish and the resultant cells were cultured in serum-free medium with 8 supplements.
GMC-N and GMC-U cells were polygonal and typical epithelial cells, but GMC-Y cells were smaller and rounder than GMC-N or GMC-U cells and tended to pile up.
The doubling times of GMC-N, U, Y cells were 18.3, 41.9, 18.2 hr, respectively.
Estrogen receptor (ER) and progesterone receptor (PgR) were measured by whole cell uptake method. Both GMC-N and GMC-U cells were ER-and PgR-positive, while GMC-Y cells were ER-positive, but PgR-negative. Estradiol-17β stimulated the growth of all these cell lines at 10-10M concentration.
All of three cell lines were tumorigenic to athymic nude mice and solid tumors developed 3 weeks after subcutaneous injection in all mice. Histological examination of the grafted tumors revealed the similar morphology with minute cavities in all cases.
These results indicate that these three established cell lines could be useful in studying the biological properties of breast carcinoma cells and the mechanisms of drugs used in endocrine therapy.
