The osseomucoid was treated with protease (Nagarse) and hyaluronidase (Sprase), and the change was investigated on the filter paper electrophoresis followed by the dyeing method with Amidoschwarz and toluidine blue and also by the periodic acid-Schiff reaction.
Both of Nagarse and Sprase gave somewhat larger mobilities to the three fractions in the osseomucoid, i, e., chondroitin sulfuric acid, protein (or neutral glycoprotein) and a com-pound of the above two components.
The spot, probably insoluble, remaining at the original point which is observed with the control sample (untreated with enzymes) disappears when treated with Nagarse, while with Sprase it lingers strongly and never be removed.
Amidoschwarz positive spot is dyed weakly after the treatment with Nagarse but the toluidine blue positive spot is not so even after the treatment with hyaluronidase.
The change on the typical figure of the spot supposed as chondroitin sulfuric acid is fairly affected by hyaluronidase.
The periodic acid-Schiff reaction did not dye the fraction supposed as chondroitin sulfuric acid but colored the almost same part dyed with Amidoschwarz and its coloration decreased remarkably by Nagarse, but little effect was observed by hyaluronidase.
Department of Oral Hygiene
