INHIBITION OF HERPES VIRUS ADSORPTION BY TWO FRACTIONS OBTAINED FROM COMMERCIAL PROTAMINE SULFATE
1971 年 18 巻 1 号 p. 39-50
詳細
1971 年 18 巻 1 号 p. 39-50
The commercial protamine sulfate could be separated into two fractions by Sephadex G-50 column which inhibited plaque formation by herpes simplex virus when cell monolayers were treated with them before adding the virus. One of them (Fr. I) contains high molecular weight proteins and the other (Fr. II) contains only protamine molecules. The former is more effective than the latter. The inhibitory effect disappears after incubation of the fractions with a large amount of cells. Both fractions do not directly reduce virus infectivity. Furthermore, they do not inhibit virus replication after virus adsorption. The virus particles remaining unadsorbed to cells pretreated with the fractions could be detected only when virus diluted in Earle's saline containing skim milk and lactalbumin hydrolysate was used for inoculation. These findings gave a clear evidence that protamine (Fr. II) and also high molecular weight proteins in Fr. I adsorb to cell surface or receptor and in consequence inhibit virus attachment to the cell surface resulting in the reduction of plaque formation.
