IN VITRO MARKER TEST OF POLIOVIRUS ON THE ESTABLISHED CELL LINES

Abstract

Within the past several years, studies have been made of at least seven markers of poliovirus which can be determined by tissue culture, physical property and serological method, and which tend to discriminate between neurovirulent and attenuated strains.Strains possessing the d⁺ marker grow more rapidly than d^-strains at low bicarbonate concentration under agar ; strains possessing the MS⁺marker grow more rapidly on the monkey kidney stable cell line than MS^-strains ; strains possessing the T⁺marker grow readily at 40°C in contrast to the T^-strains ; Cf antigen of strains possessing the CFt⁺marker are more resistant than CFt^-strains at various temperatures ; strains possessing the A⁺marker grow more rapidly on the primary human amnion cell than A^-strain ; strains possessing the E+marker elute more rapidly from an DEAE column than E^-strain.The most promising advance in the identification of virus strains is the intratypic serodifferentiation test investigated by McBride, Gard and Wecker.However, in these studies they observed principally the change in the primary monkey kidney cells, without touching on the established cell lines.This paper will be concerned chiefly with the d, T, MS, and CFt marker test on the established cell lines.