SOME ADDITIONS TO THE URINE PRECIPITIN REACTION FOR SCHISTOSOMIASIS JAPONICA

Abstract

Okabe and Tanaka (1958) reported the urine precipitin reaction for schistosomiasis japonica in 1958. In the field studies the authors sometimes met negative reaction among school children. A teacher asked children to bring the first morning urine for test. When children forgot the first urine, they might have brought the second or third urine specimen to our experiment. Experiments were conducted to examine the entire day's urine of schistosomiasis patients. At first sodium azide was used for the preservation of urine. This chemical is recently difficult to get, so merthiolate (Japanese name, Merzonin) was employed. Immunization of rabbits with the schistosomiasis patient's urine was tried in order to prove the presence of the antigen in the urine and to obtain antiserum for Schistosoma japonicum antigen.