抄録
Cypoviruses are insect viruses that produce a form of progeny in which virions are embedded within a crystalline particle called the polyhedron. In this study, we demonstrated that the N-terminus, but not the C-terminus of the virion structural protein, VP3, functions as an "occlusion signal", which can direct the stable incorporation of foreign proteins into polyhedra. A VP3-EGFP fusion protein was shown to be uniformly occluded within the polyhedra, to be detectable at the surface of these particles. A broader survey revealed that the VP3 occlusion signal could direct the incorporation of foreign proteins into polyhedra. Immune reactivity were detected on the surface of polyhedra containing occluded foreign proteins, when these particles were arrayed on a glass slide by laser fabrication.
