抄録
SATOGEN, a soil regeneration material, is made by low-temperature fermentation
of pruned branches, dredged soil, and mushroom waste mycorrhizal beds, which suppresses
plant diseases and promotes fertilization at low temperatures. The bacterial flora of
satogen was analyzed with the aim of identifying the bacterial species responsible for these
functions. PCR-denaturing gradient gel electrophoresis (DGGE) analysis revealed one
major band. In addition, next-generation sequencing analysis revealed a characteristic
bacterial flora. Compared to the flora of the dredged material, the flora of SATOGEN was
6.8 times higher in Archaea (3.4%), 0.56 times higher in Proteobacteria (39.4%), 3.2 times
higher in Actinobacteria (23.8%), 3.4 times higher in Firmicutes (15.0%), 3.5 times higher
in Chloroflexi (5.2%). At the genus level, Firmicutes phylum Bacillus was the most
abundant genus, accounting for 6.2% of the total. The major band detected by PCR-DGGE
analysis is probably that of the genus Bacillus. The genera that account for more than 1% of
the total number of bacteria are Lysobacter, Streptomyces, Actinomadura, and Flexibacter,
which produce antibiotics, and Lysobacter, Microbispora, Bacillus, Cytophaga, and
Acidobacterium, which degrade chitin. These genera of bacteria can be expected to suppress
plant diseases.
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