抄録
A high performance liquid chromatography and mass spectrometry (HPLC/MS) combination system with a self-spouting and vacuum nebulizing assisted interface was applied to measure the chemical ionization (CI) mass spectra of free- and PTH- amino acids. An aliquot of the injected sample solution carried to the interface with flow of a molbile phase (100 μl/min) was nebulized under a reduced pressure, and introduced into an ion source where the vapour of the mobile phase was used as a reagent gas for CI. The CI mass spectra of twenty kinds of free amino acids were measured using water as a mobile phase. The mass spectra for fifteen kinds of PTH amino acids which eluted successively from a separation column were also obtained using a mixture of acetonitrile and water of 3:1 (v/v) ratio as a mobile phase.
