抄録
Urinary leukotriene E4 (LTE4) levels in healthy volunteers were determined using liquid chromatography/tandem mass spectrometry (LC/MS-MS). LTE4 and its internal standard were extracted by simple solid-phase extraction and analyzed using LC/MS-MS in the selected reaction monitoring (SRM) mode by monitoring the transitions between m/z 438 and m/z 333 for LTE4 and between m/z 441 and m/z 336 for LTE4-d3. We detected LTE4 (64.7±8.3 pg/mg creatinine, n=13) in healthy human urine. Then, we determined the intra- (within one day) and inter-day (for two weeks) variation of the excretion rates of LTE4 in healthy volunteers (n=5). No significant variations in urinary LTE4 excretion rates were observed in both examinations. In conclusion, LC/MS-MS is a rapid, accurate and sensitive method for determination of urinary LTE4. The present results about urinary LTE4 levels and variation of LTE4 excretion in healthy volunteers can be helpful in evaluating the role of cysteinyl LTs in pathophysiological conditions.
