Characterization of Gd₂O₃:Eu³⁺ Nanocomplexes Conjugate with IgG for the Identification of CEA Tumor Cells

Abstract

In this paper, the Gd₂O₃:Eu³⁺ nanospheres with the ratio Eu³⁺/Gd³⁺ of 6% mol were successfully synthesized by a multi-step chemical method with the mean nanoparticles size of about 200 to 220 nm and a low standard deviation. The morphology, structure and optical behavior of as-synthesized and annealed Gd₂O₃:6%Eu³⁺ samples were investigated by field-emission scanning electron microscopy (FESEM), X-ray diffractions (XRD), and photoluminescence spectroscope (PL) The luminescence lifetime of samples was also investigated and discussed. The biomedical nanocomplexes of Gd₂O₃:6%Eu³⁺ with IgG were fabricated by glutaraldehyde linking after covering nanospheres with amino Silica. The luminescent nanocomplexes Gd₂O₃:6%Eu³⁺@Silica-NH₂-IgG with different concentrations have been incubated with CEA tumor cells to investigate and evaluate the detection capacity by using a fluorescence optical microscopy for the early detection and treatment of cancer.