抄録
Recently, “synthetic biology” has effectively produced valuable compounds by introducing biosynthetic enzyme genes into microorganisms such as Escherichia coli and yeast. Some useful drugs were successfully produced in microorganisms, however, growth retardation and low productivity have been reported in some cases, which is mainly attributed to negative feedback inhibition and the presence of metabolic bottlenecks. Transport engineering has emerged as an effective method to address these challenges in the establishment of microbial production. In this review, we highlight the recent examples of transport engineering in microorganisms producing benzylisoquinoline alkaloids and discuss future studies for the effective production of valuable metabolites through the integration of coculture and transport engineering.
