Running Colour Electrophoresis and Silver Nitrate Doublestaining of Polyacrylamide Gel

Abstract

A running colour electrophoresis of protein in sodium dodecylsulfate (SDS)-polyacrylamide gel (PAG) has been developed and the coloured gel was restained with silver nitrate and hydroquinone (HQ) within 20min. Trichloroacetic acid (TCA) was found to be a powerful destaining reagent and reproducibility of this double staining procedure was excellent.