衛生動物
Online ISSN : 2185-5609
Print ISSN : 0424-7086
ISSN-L : 0424-7086
アカイエカ類の糖類摂食性試験の一手段
渡辺 護上村 清久保田 憲太郎
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ジャーナル フリー

1973 年 24 巻 1 号 p. 17-21

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A simplified technique was devised for the analysis of various sugars in adult mosquitoes. The sugar meal in the diverticulum of mosquitoes was applied directly on a filter (Toyo No. 51 A) and the ordinary one-dimensional paper-partition chromatography was carried out with standard sugar solutions. The results obtained by this simplified method were as acurate as that obtained by the previously reported and more complicated ones, by homogenizing the samples, concentrating it and developing on the filter paper. Moreover, the procedure is very simple and many samples can be tested within a short period of time, and thus it can probably be used to the field experiments. Employing this procedure, sugar preference tests were carried out with Culex pipiens pallens and C.p. molestus. Lactose was least preferred among the sugars examined and there was no significant difference in preference among fructose, glucose, sucrose and maltose. It was also observed that the hydrolysis of sugars began 3 hours after the intake. Detection of disaccharide become almost impossible 4 hours after the intake and sugars disappeared after 8 hours indicating the complete digestion and absorption of these sugars.
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© 1973 日本衛生動物学会
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