The effect of the juvenile hormone analogue, methoprene, as an oviposition regulator was examined using an autogenous mosquito Culex pipiens molestus. Either when the mosquitoes were fed on methoprene with sugar solution or when they were exposed to filter paper treated with methoprene, follicle maturation was varied and oviposition was inhibited. Moreover, it was confirmed that methoprene applied topically at 0.01μg/female was affected reproduction. Upon dissection the females which had oviposited were seen to have eggs retained in their ovaries, whereas those which had not oviposited contained follicles at various stages of maturation.