Molecular Science
Online ISSN : 1881-8404
Award Accounts
赤外分光法による膜タンパク質の分子機構研究
古谷 祐詞
著者情報
ジャーナル オープンアクセス

2014 年 8 巻 1 号 p. A0067-

詳細
抄録

Membrane proteins are indispensable for cell signaling, bioenergetics, and transportation. Atomic structures of membrane proteins have been elucidated by methods in structural biology (e.g. X-ray crystallography). Structural changes of them in action should be investigated to improve the understanding of the molecular mechanisms in more detail. Infrared spectroscopy is a powerful method to analyze structural changes of protein by combining it with various kinds of stimulus-induced difference spectroscopic techniques. In this review article, my recent studies on microbial rhodopsin, ion channel and ion pump proteins are summarized. Light-induced difference infrared spectroscopy revealed the hydrogen-bonding change of Thr204 in pharaonis phoborhodopsin upon photoisomerization of the retinal chromophore, which turned out to be a key residue for the signal transduction. Attenuated total reflection (ATR) method was applied on the studies of ion-protein interaction of a potassium channel, KcsA, and a sodium pump, V-type ATPase. Finally, development of a rapid-buffer exchange apparatus for time-resolved ATR infrared spectroscopy is introduced, which would be a powerful technique for investigating ion- and ligand-binding reactions of membrane proteins.

著者関連情報
© 2014 分子科学会
前の記事 次の記事
feedback
Top