Development of STS markers for the identification of Pholiota nameko cultivars and wild isolates

Abstract

Pholiota nameko is one of the popular and economically important edible mushrooms. The identification of a P. nameko cultivar is important for the protection of its producer's rights. To identify P. nameko cultivars, we have developed 7 STS (Sequence Tagged Site) markers based on RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeat) analysis. We were able to identify seven P. nameko cultivars in this study using these STS markers. We also showed that the method using these markers is applicable to the discrimination of the wild isolates of P. nameko.