Abstract
Microsatellite markers were developed for the cultivated mushroom, Pleurotus eryngii, following the analysis of 88 unique (CT)n or (GTG)n sequences. In this study, we showed that 30 P. eryngii strains, comprising five cultivars and 25 wild strains, could be identified using a total of 20 simple sequence repeat (SSR) markers, and a combination of only six selected SSR markers was sufficient to discriminate these strains. These SSR markers are a very useful tool for the precise identification of P. eryngii strains, allowing for protection of the rights of the original producer.