Abstract
The effect of soba-shochu distillery waste (SDW) as a nutritional additive in liquid medium used for the production of benzaldehyde (BAH) by Grifola gargal in comparison with yeast extract and polypeptone was investigated. When yeast extract and polypeptone in PGY medium (polypeptone, yeast extract, and glucose) were replaced by a diluted SDW solution, the mycelial weight and BAH production by G. gargal increased about 3 and 2 fold, respectively. However, the addition of 1% glucose to SDW was necessary for BAH production. In medium optimized for BAH production (SDW+1% glucose), the levels of ergothioneine (EGT) per mycelial dry weight were similar to those in PGY medium, but the total yield of EGT from a liquid batch culture increased about three fold because of the increased mycelial weight of G. gargal. Together, these results show that SDW can be used as a nutritional additive for increasing BAH and EGT production by liquid cultures of G. gargal.
