Abstract
During the cultivation of Lentinula edodes, brown film was formed; however, the microbiome associated with brown film has not been examined. We investigated bacterial communities and attempted to find potential bacterial biomarkers on the brown film for distinguishing different flushes of L. edodes cultivation. In bacterial composition, Firmicutes was a dominant bacterium in the first flush, and Proteobacteria was a dominant bacterium in the third flush (last flush). The first flush group showed a significant difference in the structure of bacterial communities compared to the third flush group. In biomarker screening, Bacillus was selected in the first flush group, and Burkholderia-Caballeronia-Paraburkholderia and Sphingomonas were selected in the third flush group by linear discriminant analysis. These findings suggested that Bacillus, and Burkholderia-Caballeronia-Paraburkholderia and Sphingomona are potential bacterial biomarkers for determining early stage and late stage of L. edodes cultivation, respectively.
