Determination of Luteoskyrin in Grain by High Perfomance Liquid Chromatography(HPLC)

Abstract

A sensitive analytical method for two mycotoxins, luteoskyrin and rubroskyrin, was developed by using reverse phase HPLC. Luteoskyrin and rubroskyrin were eluted from ODS column with CH³CN :H²O (40:60) containing 0.5% CH³COOH. Detection was efficiently made by visible absorption at 429 nm. Neither ultraviolet detection at 254 nm nor fluorescence detection gave better result. Detection limit was about 10 ppb when the method was applied to rice sample.