再構成チトクロームP-450系によるマイコトキシンの生転換
1985 年 1985 巻 22 号 p. 28-30
詳細
1985 年 1985 巻 22 号 p. 28-30
Metabolic transformation of ochratoxin A (OA) and emodin (EM) was investigated in the microsomal and reconstituted cytochrome P-450 systems of the rat liver. In the microsomal system, OA was transformed into 4 (R)- and 4 (S)-4-hydroxy-OAs, and 3-methylcholanthrene (3 MC) as well as PCB enhanced the microsomal hydroxylation of OA to 4 (R)-4-hydroxy-OA. In the reconstituted cytochrome P-450 system, cytochrome P-450 II-a (maximal CO-differential spectrum at 448.0 nm and high-spin form), fractionated from PCB-microsomes, selectively catalyzed the hydroxylation of OA and EM into 4 (R)-4-hydroxy-OA and 2-hydroxy-EM, respectively.
