抄録
In vitro culture cell line, Kagura-1, was established from a rat hepatocellular carcinoma induced by aflatoxin B1 (AFB1). When the cells at passage 15 were cultured in Dulbecco's modified Eagle medium supplemented with 10% fetal calf serum in collagencoated dishes, the population doubling time and saturation density were 13.2 hr and 1.9×105 cells/cm2, respectively. The established cells formed colonies in soft agar.We examined the expression of proto-oncogenes in two liver tumors induced by AFBI and Kagura-1 cell. Both c-myc and c-Ha-ras expressions were activated at high levels in both tumors and Kagura-1 cells.
