A case report on the high level contamination of afatoxins B1 and B2 in domestic peanuts

T. TAMAKA; A. HASEGAWA; N. TOYAZAKI; Y. NATSUKI; Y. MATSUDA; S. YAMAMOTO; S. UDAGAWA; Y. UENO

doi:10.2520/myco1975.1986.47

A case report on the high level contamination of afatoxins B₁ and B₂ in domestic peanuts

T. TAMAKA, A. HASEGAWA, N. TOYAZAKI, Y. NATSUKI, Y. MATSUDA, S. YAMAMOTO, S. UDAGAWA, Y. UENO

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1986 Volume 1986 Issue 23 Pages 47-52

DOI https://doi.org/10.2520/myco1975.1986.47

[Correction] Proc. Jpn. Assoc. Mycotoxicol. Vol.1986 No.24 (1986) pp.68-68

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Abstract

Contamination by aflatoxins B₁ and B₂ in shelled peanuts harvested from Shizuoka Prefecture, Japan, was investigated. The extraction with methano1-1% NaCl solution (55:45, v/v), followed by purification on a Sep-Pak silica cartridge column, and HPLC analysis revealed the contamination of 5.9mg/kg of aflatoxin B1 and 0.3mg/kg of aflatoxin B₂ in the shelled peanuts. Aflatoxin B₁ was confirmed by mass spectral analysis. Mycological survey detected aflatoxigenic strains of Aspergillus flavus on peanut kernels in the infested lot.

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