サンドイッチELISAによるアフラトキシンB_l-ヒト血清アルブミン付加体測定

抄録

Employing monoclonal antibody (mAb) AL. 2 reactive for aflatoxin B₁-lysin adducts (AFB₁-Lys), biotin-conjugated affinity purified anti-human serum albumin (HSA) antibody, and streptoavidin-conjugated horseradish peroxidase (HRP), the sandwich ELISA (sELISA) AFB₁-HSA adducts was constructed. In this sELISA, AFB₁-HSA adducts in crude albumin fraction were detected as low as 5pg AFB₁-Lys equivalent/mg HSA without pronase digestion. Since the present sELISA is not requested for an enzymatic hydrolysis of HSA, more than 150 serum samples could be analyzed in one week. AFB₁-HSA analysis of 70 human sera sampled during 1986-1994 in Haimen (China), a high endemic area of primary liver cancer (PLC), revealed that 20-70% of the sera were positive for AFB₁-HSA adducts. The case-control study on 137 sera (69 controls and 68 cases of PLC) collected in 1994 revealed no significant difference both in the positivity and the average level. As for 100 sera (44 controls and 56 cases) sampled in 1995, the postivity for AFB₁-HSA in the cases was significantly higher than that in the controls, although no difference in their contents was observed.