Detection of genetic variation among single-spore isolates and identification of genets of Armillaria ostoyae by AFLP analysis with Texas Red^TM-labeled selective primer

Abstract

AFLP (amplified fragment length polymorphism) analysis was applied to Armillaria ostoyae isolates (single-spore isolates and field isolates from the same forest). For detection of AFLP, we have developed a modified method using DNA sequencer with Texas Red-labeled selective primer. In analysis of single-spore isolates, this technique provided large numbers of highly polymorphic DNA markers, which can be used to identify genets. The results suggested that outbreeding might be common in A. ostoyae.