Differential expression of two gpd genes in the cultivated mushroom Pleurotus eryngii using RNA sequencing analysis

Junjun Shang; Ruiheng Yang; Lihua Tang; Yán Li; Yan Li; Wenjun Mao; Ming Gong; Ying Wang; Yoichi Honda; Dapeng Bao

doi:10.1016/j.myc.2019.06.004

Note

Differential expression of two gpd genes in the cultivated mushroom Pleurotus eryngii using RNA sequencing analysis

Junjun Shang, Ruiheng Yang, Lihua Tang, Yán Li, Yan Li, Wenjun Mao, Ming Gong, Ying Wang, Yoichi Honda, Dapeng Bao

Author information

Junjun Shang
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences
Ruiheng Yang
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences
Lihua Tang
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences
Yán Li
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences
Yan Li
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences
Wenjun Mao
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences
Ming Gong
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences
Ying Wang
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences
Yoichi Honda
Laboratory of Forest Biochemistry, Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University
Dapeng Bao Corresponding author
Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences

Keywords: Glyceraldehyde-3-phosphate dehydrogenase (GPD), Transcriptional analysis, Promoter

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Supplementary material

2019 Volume 60 Issue 6 Pages 351-353

DOI https://doi.org/10.1016/j.myc.2019.06.004

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Abstract

Transcriptional analysis showed that Pegpd1 was constitutively and highly expressed in various developmental stages of P. eryngii. The expression level of Pegpd2 was much lower than Pegpd1. The promoter of Pegpd1 contained the conserved cis-regulatory elements and could be used to drive heterologous gene expression in genetic transformation of P. eryngii.

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