不飽和七員環化合物の實驗的並びに臨床的研究

第23報 Tropolone及びその誘導体の日本腦炎ビールスに對する試驗管内作用

抄録

In-vitro experiment was conducted on the action of tropolone (Trp) and its eight derivatives against Japanese B encephalitis virus (Nakayama strain).
The substance to be examined, dissolved in tween 80, was added to the supernatant fluid obtained by ultracentrifuging the brain emulsion of virus-inoculated mice. After incubating at 22°C for 1 hour, the mixture was intracerebrally inoculated into a fixed number of mice. Comparing the result with the control, logarithm difference (log diff.) of LD₅₀ was computed. Antiviral potency is represented by -log diff.
The result was as follows. At 1000 γ/cc the following values were registered:
Hinokitiol (Hi) (-log diff.=4.2), Hi-natrium (2.5), Trp (4.1),
3-monobromotrp (3.2), 3-5-dibromotrp (3.3), 3-7-dibromotrp (5.3),
3-5-7-tribromotrp (4.9), mercaptotropone (2.0)
3-5-7-tribromo-2-aminotropone showed no potency against virus dilutions stronger than 10^-6.
Hi, Trp, 3-7-dibromotrp and 3-5-7-tribromotrp showed considerable potency at 100 γ/cc. Even at 10γ/cc Hi and Trp registered a slight potency.
On the other hand, it was noted that the antiviral potency of 3-monobromotrp and 3-5-dibromotrp at 100 γ/cc was very weak, and that Hi-Natrium and mercaptotropone revealed no potency at 100 γ/cc.