Quantitative Real-time RT-PCR Analyses of DNA—damage-recovery-related Gene Expressions of Mouse Macrophage-like Cell Line RAW264 when Exposed to IC₅₀ Nickel Ions

抄録

The primary purpose of this study was to analyze the effects of IC₅₀ nickel (Ni) (2+) ions on expressions of seven DNA-damage-recovery genes (Mre11a, Dclre1a, Ddb1, Msh3, Rad51, Ercc1 and Xpa) of mouse macrophage-like cell line RAW264 cultured for 4h and 24h. Quantitative real-time RT-PCR analyses revealed that IC₅₀ Ni (2+) ions significantly down-regulated expressions of these seven genes with time. Additional analyses clarified that IC₅₀ Ni (2+) ions up-regulated expression of two cell-protection genes (Hmox1 and Ier3) and one anti-metal-toxicity gene (MT1) of RAW264 cells with time. Taken together, our data suggested that excessive (i.e. IC₅₀ concentration) Ni (2+) ions overcame intrinsic cell protective mechanisms of RAW264 cells, and inhibited productions of DNA-damage-recovery genes, leading to reduced cell viabilities.